Bioremediation of hexavalent chromium by transformation of Escherichia coli DH5α with chromate reductase (ChrR) genes of Pseudomonas putida isolated from tannery effluent.

Aims: Hexavalent chromium Cr(VI), a toxic heavy metal, is a serious pollutant of tannery effluent, and its accumulation in soil and water causes severe environmental concerns of increasing public health issues. The present study focus on the isolation and identification of chromium-reducing bacteria collected from the tannery industry in Dindigul, Tamil Nadu. Chromium-reducing bacteria Pseudomonas putida were identified by 16S rRNA sequencing followed by BLAST search. The plasmid with Cr(VI) reductase gene was isolated from Pseudomonas putida and transferred to E. coli DH5α for further studies.

Methods And Results: The bacterial cultures were kept under controlled conditions for 72 h to observe the growth rates and bacterial resistance to chromium. When strains wild type and transformant E. coli DH5α were grown in chromium supplemented media revealed significant growth, but strains cured type Pseudomonas putida and E. coli DH5α were minimum growth. The Cr(VI) reduction employed by transformant E. coli DH5α and wild Pseudomonas putida was 42.52 ± 1.48% and 44.46 ± 0.55%, respectively. The culture supernatant of the wild Pseudomonas putida and transformant E. coli DH5α showed an increased reduction of Cr(VI) compared to cell extract supernatant and cell debris due to the extracellular activity of chromium reductase has been responsible for Cr(VI) reduction. Besides, the chromium reductase gene was confirmed in the isolated Pseudomonas putida and transformant E. coli DH5α.

Conclusions: Transformant bacteria could employ an alternative method for heavy metal detoxification in contaminated environments like tannery effluent and mining processes.