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Dye labeling for optical imaging biases drug carriers' biodistribution and tumor uptake. | LitMetric

Dye labeling for optical imaging biases drug carriers' biodistribution and tumor uptake.

Nanomedicine

Institute for Experimental Molecular Imaging, RWTH Aachen University, Forckenbeckstrasse 55, 52074 Aachen, Germany; Helmholtz Institute for Biomedical Engineering, RWTH Aachen University, Aachen, Germany; Fraunhofer MEVIS, Institute for Medical Image Computing, Aachen, Germany. Electronic address:

Published: February 2023

AI Article Synopsis

  • This study investigates how different near-infrared fluorescent dyes affect the biodistribution and tumor cell uptake of nanocarriers in A431 tumor-bearing mice.
  • It finds that hydrophobic dyes show higher uptake in tumors compared to hydrophilic dyes, with their elimination influenced by hydrophobicity and blood circulation times.
  • The research concludes that dye labeling impacts how nanocarriers accumulate in tumors, suggesting that low-interference dyes like AF750 and IRDye750 are better suited for labeling hydrophilic nanocarriers to obtain unbiased results.

Article Abstract

Biodistribution analyses of nanocarriers are often performed with optical imaging. Though dye tags can interact with transporters, e.g., organic anion transporting polypeptides (OATPs), their influence on biodistribution was hardly studied. Therefore, this study compared tumor cell uptake and biodistribution (in A431 tumor-bearing mice) of four near-infrared fluorescent dyes (AF750, IRDye750, Cy7, DY-750) and dye-labeled poly(N-(2-hydroxypropyl)methacrylamide)-based nanocarriers (dye-pHPMAs). Tumor cell uptake of hydrophobic dyes (Cy7, DY-750) was higher than that of hydrophilic dyes (AF750, IRDye750), and was actively mediated but not related to OATPs. Free dyes' elimination depended on their hydrophobicity, and tumor uptake correlated with blood circulation times. Dye-pHPMAs circulated longer and accumulated stronger in tumors than free dyes. Dye labeling significantly influenced nanocarriers' tumor accumulation and biodistribution. Therefore, low-interference dyes and further exploration of dye tags are required to achieve the most unbiased results possible. In our assessment, AF750 and IRDye750 best qualified for labeling hydrophilic nanocarriers.

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Source
http://dx.doi.org/10.1016/j.nano.2023.102650DOI Listing

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