Characterisation of the heat shock protein Tid and its involvement in stress response regulation in .

The impact of various environmental stresses on native fitness has attracted intense attention in China. However, the defence responses of to different stressors are poorly understood. Here, we aimed to elucidate the regulatory mechanism mediated by the tumorous imaginal discs (Tid) protein of (AccTid) in response to stressors. We used some bioinformatics softwares to analyse the characterisation of Tid. Then, qRT-PCR, RNA interference and heat resistance detection assays were used to explore the function of Tid in stress response in . is a homologous gene of human Tid1 and Tid56, contains a conserved J domain and belongs to the heat shock protein DnaJA subfamily. The level of induced expression was increased under temperature increases from 40°C to 43°C and 46°C, and knockdown decreased the heat resistance of , indicating that the upregulation of plays an important role when is exposed to heat stress. Interestingly, contrary to the results of heat stress treatment, the transcriptional level of was inhibited by cold, HO and some agrochemical stresses and showed no significant change under ultraviolet ray and sodium arsenite stress. These results suggested that the requirement of for Tid differs markedly under different stress conditions. In addition, knockdown of increased the mRNA levels of some Hsps and antioxidant genes. The upregulation of these Hsps and antioxidant genes may be a functional complement of knockdown. plays a crucial role in stress responses and may mediate oxidative damage caused by various stresses. Our findings will offer fundamental knowledge for further investigations of the defence mechanism of against environmental stresses.