It has been approved that one of the most dangerous foodborne pathogenic bacteria is O157:H7, which is responsible for several infection and death cases worldwide. It is well documented that in the developing countries O157:H7 is considered the main causative pathogen of human gastrointestinal infections. Therefore, the current research was aimed to evaluate the prevalence of O157:H7 in dairy cattle's milk using a rapid method, in Iraq (Najaf, Baghdad, Kirkuk, and Erbil). Over a period of 6 months (During hot months) samples were obtained and investigated by culturing on selective media (CT-SMAC). The multiplex PCR (m-PCR) also used for milk sample direct investigation. Using biochemical tests the recorded data showed that, 2 recognized isolates were , while the recorded data obtained from m-PCR assay revealed that none of the isolated was toxigenic E.coli O157:H7. The results of m-PCR on the milk samples revealed that 45 milk samples contained at least one of the following genes: genes. Also the results of the m-PCR revealed that 2 samples (raw milk) were toxigenic O157:H7 positive. In conclusion, to the best of authors' knowledge, this investigation was the first report on the prevalence of O157:H7 in the raw milk samples in Iraq. The results showed that the proportion of contaminated milk samples contaminated with O157:H7 identified in the current survey were similar to that the results of the previously published research from different dairy products across different countries in the Middle East region.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9759260 | PMC |
| http://dx.doi.org/10.22092/ARI.2022.358832.2317 | DOI Listing |
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