AI Article Synopsis
- Scientists successfully isolated and characterized a new virulent phage called Bfsp1, targeting the bacterium Cytobacillus firmus.
- Bfsp1 has a linear, double-stranded DNA genome of 22,320 base pairs with a GC content of 36.06% and features 10-bp inverted terminal repeats, containing 33 open reading frames.
- Phylogenetic and comparative genome analyses establish Bfsp1 as a distinct and novel member of the Salasmaviridae family.
Article Abstract
We isolated, identified, and characterised Bfsp1, a novel virulent phage of Cytobacillus firmus. Morphologically, Bfsp1 is similar to phi29-like phages. The linear, double-stranded DNA genome of Bfsp1 is 22,320 bp in length, has a GC content of 36.06%, and has 10-bp inverted terminal repeats. The genome contains 33 open reading frames, and functions of 15 of them were predicted. Comparative genome analysis showed that Bfsp1 is distinct from other known phages, and this was confirmed by phylogenetic analysis. Morphological, genomic, and phylogenetic data indicated that Bfsp1 is a novel member of the family Salasmaviridae.
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| http://dx.doi.org/10.1007/s00705-022-05660-y | DOI Listing |
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