Genome-Wide Analysis of the Gene Family in and Functional Analysis of in Root Growth and Amino Acid Transport.

Int J Mol Sci

State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of National Forestry and Grassland Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China.

Published: December 2022

The adventitious root (AR) is the basis for successful propagation by plant cuttings and tissue culture and is essential for maintaining the positive traits of a variety. Members of the amino acid/auxin permease (AAAP) gene family play indispensable roles in various plant metabolisms and have few studies on root growth and amino acid transport. In this study, with a systematic bioinformatics analysis of the AAAP family, 83 were identified from and grouped into 8 subfamilies. Subsequently, chromosomal distribution, genetic structure, cis-elements analysis, and expression pattern analysis of the AAAP family were performed and the potential gene regulating root development was screened by combining the results of RNA-Seq and QTL mapping. was proven as promoting root development by enhancing AR formation. Differentially expressed genes (DEGs) from RNA-seq results of overexpressing lines were enriched to multiple amino acid-related pathways, and the amino acid treatment to transgenic lines indicated that regulated amino acid transport, including tyrosine, methionine, and arginine. Analysis of the AAAP gene family provided a theoretical basis for uncovering the functions of AAAP genes. The identification of on root promotion and amino acid transport in will help with breeding new woody plant species with strong rooting ability.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9820651PMC
http://dx.doi.org/10.3390/ijms24010624DOI Listing

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