Temporally shaped vortex phase laser pulses for two-photon excited fluorescence.

We report temporally shaped vortex phase laser pulses for two-photon excited fluorescence of dyes. The particularly tailored pulses are generated by first utilizing a temporal pulse shaper and subsequently a two-dimensional spatial pulse shaper. Various vortex phase shaped structures are demonstrated by combining different two-dimensional phase patterns. Moreover, perpendicular polarization components are used to achieve an enhanced radial two-photon excited fluorescence contrast by applying third order phase functions on the temporal pulse shaper. Particularly, the spatial fluorescence structure is modulated with a combination of Gaussian and vortex phase shaped pulses by modifying only the phase on the temporal modulator. Thereby, interference structures with high spatial resolution arise. The introduced method to generate temporally shaped vortex phase tailored pulses will provide new perspectives for biophotonic applications.