AI Article Synopsis
- Low temperatures can harm how plants grow, so scientists studied cold-resistant genes in Caucasian clover to help them survive.
- They found a gene that is important for fighting cold stress and helps the plant remove harmful substances and boost its defenses.
- This research could help in breeding better plants that can handle harsh conditions like cold, salt, and drought.
Article Abstract
Low temperature (LT) is an important threat to the normal growth of plants. In this study, based on the full-length transcriptome sequencing results, the cold resistance genes were cloned from Caucasian clover with strong cold resistance. We cloned the CDS of , which is 1311 bp in length and encodes 436 amino acids. The molecular weight of the protein is 48.97 kDa, which had no transmembrane structure, and its isoelectric point (pI) was 5.42. We predicted the structure of and found 29 phosphorylation sites. Subcellular localization showed that was localized and expressed in cell membrane and chloroplasts. The gene was induced by stress due to cold, salt, alkali and drought and its expression level was higher in roots and it was more sensitive to LT. Analysis of transgenic A. thaliana plants before and after LT treatment showed that the gene enhanced the removal of excess HO, and increased the activity of antioxidant enzymes, thus improving the plant's ability to resist stress. Additionally, the OE lines showed increased cold tolerance by upregulating the transcription level of cold-responsive genes (, , , , , and ). This study demonstrates that is actively involved in the responses of plants to LT stress. We also provide a theoretical basis for breeding and a potential mechanism underlying the responses of Caucasian clover to abiotic stress.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9809470 | PMC |
| http://dx.doi.org/10.3389/fpls.2022.968965 | DOI Listing |
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