Polymers of biological origin have become a topic of interest due to growing concerns about the environmental impact of the disposal of plastics. In recent years, the production of ecobenign microbial polymer polyhydroxyalkanoates (PHAs) using inexpensive and renewable resources has gained significant interest as these compounds are highly biodegradable, biocompatible, and sustainable. This study used leaf endophytic isolate RCL 02, obtained from the oil-yielding plant L., to achieve statistical optimization of culture variables for the enhanced production of PHAs utilizing sugarcane molasses as the sole carbon source. A three-level and four-factor Box-Behnken design of response surface methodology was implemented to optimize the process variables, namely molasses (carbon substrate), ammonium sulfate (nitrogen source), initial pH, and incubation period, for improved biomass formation and PHA production. The highest growth (14.8 g/l) and PHA production (85.2%, dry cell weight) by the isolate were observed with 47 g/l molasses, 3 g/l ammonium sulfate, an initial pH of 6.7, and 62 h of incubation. Statistical optimization of the process allowed achieving a 1.6-fold increase in the PHA yield (7.8-12.6 g/l) compared with the conventional single-factor system of analysis. The biopolymer thus produced was confirmed as a copolymer of 3-hydroxybutyrate and 3-hydroxyvalerate [P(3HB-co-3HV)] using H nuclear magnetic resonance spectroscopic analysis and was found to contain 7.8 mol% 3-hydroxyvalerate. These findings clearly indicate the efficacy of the RCL 02 isolate in the biotransformation of raw sugarcane molasses to P(3HV-co-3HV), without the need for supplementation with high-cost precursors.
Download full-text PDF |
Source |
---|---|
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9642960 | PMC |
http://dx.doi.org/10.5114/bta.2022.118671 | DOI Listing |
Foods
November 2024
Department of Bioprocess Engineering and Biotechnology, Polytechnic Center, Federal University of Parana, Rua Cel. Francisco H. dos Santos-100, Curitiba 81530-000, PR, Brazil.
Thraustochytrids are emerging as a valuable biomass source for high-quality omega-3 polyunsaturated fatty acids (PUFAs), crucial for both human and animal nutrition. This research focuses on cultivating SR21 using cost-effective agro-industrial by-products, namely sugarcane molasses (SCM), corn steep liquor (CSL), and residual yeast cream (RYC), to optimize biomass and lipid production through a comprehensive multistep bioprocess. The study involved optimization experiments in shake flasks and stirred-tank bioreactors, where we evaluated biomass, lipid content, and DHA yields.
View Article and Find Full Text PDFSci Rep
November 2024
College of Biological and Chemical Engineering, Guangxi University of Science and Technology, Liuzhou, 545006, People's Republic of China.
Sugarcane molasses is an ideal economical raw material for ethanol production because of its wide availability, low cost and nutrient content. However, benzoic acid compounds with toxic effects on yeast cells are commonly found in sugarcane molasses. At present, the molecular mechanism of the toxic effects of benzoic acid on Saccharomyces cerevisiae has not been elucidated.
View Article and Find Full Text PDFBioresour Technol
February 2025
Faculty of Chemistry, Adam Mickiewicz University in Poznań, Uniwersytetu Poznańskiego 8, 61-614 Poznań, Poland.
Crude sugarcane molasses (SCM) was successfully applied for the first time as a bio-feedstock for producing biochar catalysts for glycerol upgrading. Preparation methods were developed, including partial or hydrothermal carbonization (abbr. PC and HTC) and chemical activation.
View Article and Find Full Text PDFJ Agric Food Chem
November 2024
State Key Laboratory of Materials-Oriented Chemical Engineering, College of Food Science and Light Industry, Nanjing Tech University, Nanjing 211816, China.
Cane molasses, a sugar-rich agro-industrial byproduct, was used to enhance the production of ε-poly-l-lysine (ε-PL) in PD-1 as a cost-effective carbon source. The sucrose metabolism pathway was engineered by heterologously expressing sucrose-6-phosphate hydrolase from W. The optimization of various promoters identified the SP44 promoter, increasing the total sugar utilization rate by 2.
View Article and Find Full Text PDFJ Air Waste Manag Assoc
January 2025
Department of Environmental Health Engineering, Faculty of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
This study focused on the feasibility of identifying and recycling inorganic phase-change materials (PCMs) from sugar industry wastes in two cities of Qazvin and Hamadan in Iran. In this study, dry sugar beet pomace, sugar beet pomace, sugar beet molasses, leaves and plant residues of sugar beet and sugarcane bagasse were investigated. The inorganic materials were identified by X-ray Diffraction (XRD), thermal characteristics were determined by differential scanning calorimetry (DSC), and morphological characteristics were determined by scanning electron microscopy (SEM).
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!