Antioxidant and antimicrobial potentials of mycelial extracts of grown in different liquid culture media.

In addition to their nutritional properties, mushrooms have emerged as a health supplement because of their medicinal potential. Many studies have shown that mushrooms exhibit important biological activities. Here, the antioxidant and antimicrobial activities of (Lév.) Singer mycelia cultivated on Sabouraud dextrose broth (SDB) and glucose peptone yeast (GPY) medium were studied. The total antioxidant status (TAS), total oxidant status (TOS), and oxidative stress index (OSI) of ethanolic extracts of mycelia were measured using Rel Assay kits. The antioxidant and oxidant potentials of mycelial extracts were determined for the first time in the present study. The highest TAS, TOS, and OSI values of were 5.416 ± 0.150 mmol/l, 1.320 ± 0.156 μmol/l, and 0.024 ± 0.003, respectively. Ethanolic mycelial extracts of showed antimicrobial activities at concentrations from 25 to 200 μg/ml against all the studied bacteria (, , , methicillin-resistant , and ) and fungi (, , and ) tested by the agar dilution method. The antifungal activity of the extract was more significant than its antibacterial activity. The antioxidant, oxidant, and antimicrobial potentials of mycelia varied depending on the culture media used. GPY medium was more suitable for the synthesis of antibiotic compounds against , while SDB medium was more appropriate for producing metabolites with antioxidant and antifungal properties. Based on the results, ethanolic extract of mycelia showed a significant pharmacological potential and could be used as a natural antioxidative and antimicrobial source for health benefit.