AI Article Synopsis
- Chikungunya fever, caused by the chikungunya virus (CHIKV) transmitted by mosquitoes, is a significant public health issue with no available vaccines or antiviral treatments.
- Researchers propose using small interfering RNA (siRNA) to silence the virus's structural and non-structural genes as a potential antiviral treatment, which can be enhanced by effective delivery systems.
- The study demonstrates that carbonated metal-organic frameworks (specifically ZIF-C) can efficiently deliver siRNAs targeting CHIKV genes, resulting in notably decreased viral replication and infectivity in infected cells compared to standard free siRNA delivery.
Article Abstract
Chikungunya fever caused by the mosquito-transmitted chikungunya virus (CHIKV) is a major public health concern in tropical, sub-tropical and temperate climatic regions. The lack of any licensed vaccine or antiviral agents against CHIKV warrants the development of effective antiviral therapies. Small interfering RNA (siRNA) mediated gene silencing of CHIKV structural and non-structural genes serves as a potential antiviral strategy. The therapeutic efficiency of siRNA can be improved by using an efficient delivery system. Metal-organic framework biocomposits have demonstrated an exceptional capability in protecting and efficiently delivering nucleic acids into cells. In the present study, carbonated ZIF called ZIF-C has been utilized to deliver siRNAs targeted against E2 and nsP1 genes of CHIKV to achieve a reduction in viral replication and infectivity. Cellular transfection studies of E2 and nsP1 genes targeting free siRNAs and ZIF-C encapsulated siRNAs in CHIKV infected Vero CCL-81 cells were performed. Our results reveal a significant reduction of infectious virus titre, viral RNA levels and percent of infected cells in cultures transfected with ZIF-C encapsulated siRNA compared to cells transfected with free siRNA. The results suggest that delivery of siRNA through ZIF-C enhances the antiviral activity of CHIKV E2 and nsP1 genes directed siRNAs.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9806579 | PMC |
| http://dx.doi.org/10.3389/fbioe.2022.1003448 | DOI Listing |
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