The genetic and molecular basis of flagellar motility has been investigated for several decades, with innovative research strategies propelling advances at a steady pace. Furthermore, as the phenomenon is examined in diverse bacteria, new taxon-specific regulatory and structural features are being elucidated. Motility is also a straightforward bacterial phenotype that can allow undergraduate researchers to explore the palette of molecular genetic tools available to microbiologists. This study, driven primarily by undergraduate researchers, evaluated hundreds of flagellar motility mutants in the Gram-negative plant-associated bacterium Agrobacterium fabrum. The nearly saturating screen implicates a total of 37 genes in flagellar biosynthesis, including genes of previously unknown function.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9812332 | PMC |
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0279936 | PLOS |
BMC Plant Biol
December 2024
Plant Production Department, College of Food and Agriculture Sciences, King Saud University, Riyadh, 11451, Saudi Arabia.
Wheat (Triticum aestivum L.), a vital crop constituting approximately 20% of global caloric intake, faces significant threats from heavy metal contamination, particularly cadmium (Cd) and chromium (Cr), along with drought stress, jeopardizing global food security. This study aimed to investigate the combined effects of these stressors and the potential of plant growth enhancers such as gibberellic acid (GA3), biochar (BC), and rhizobacteria to improve wheat growth.
View Article and Find Full Text PDFMicroorganisms
September 2024
USDA-ARS Crop Improvement and Genetics, Western Regional Research Center, Albany, CA 94710, USA.
Citrus is one of the world's most important and widely produced fruit crops, with over a 100 million metric tons harvested from nearly 10 million hectares in 2023. Challenges in crop maintenance, production, and fruit quality necessitate developing new traits through Agrobacterium-mediated genetic transformation. While a few strains (EHA105, GV3101, LBA4404) are known to transform citrus, many wild strains remain untested.
View Article and Find Full Text PDFEcotoxicol Environ Saf
October 2024
SUBATECH, IMTA/CNRS-IN2P3/Université de Nantes, 4, rue Alfred Kastler, 44304 Nantes, France; LTSER "Zone Atelier Territoires Uranifères", Clermont-Ferrand, Aubière F-63000, France. Electronic address:
Diatoms and bacteria play a vital role in investigating the ecological effects of heavy metals in the environment. Despite separate studies on metal interactions with diatoms and bacteria, there is a significant gap in research regarding heavy metal interactions within a diatom-bacterium system, which closely mirrors natural conditions. In this study, we aim to address this gap by examining the interaction of uranium(VI) (U(VI)) with Achnanthidium saprophilum freshwater diatoms and their natural bacterial community, primarily consisting of four successfully isolated bacterial strains (Acidovorax facilis, Agrobacterium fabrum, Brevundimonas mediterranea, and Pseudomonas peli) from the diatom culture.
View Article and Find Full Text PDFJ Phys Chem Lett
August 2024
Dipartimento di Chimica e Chimica Industriale, University of Pisa, 56124 Pisa, Italy.
Bacteriophytochromes are light-sensing biological machines that switch between two photoreversible states, Pr and Pfr. Their relative stability is opposite in canonical and bathy bacteriophytochromes, but in both cases the switch between them is triggered by the photoisomerization of an embedded bilin chromophore. We applied an integrated multiscale strategy of excited-state QM/MM nonadiabatic dynamics and (QM/)MM molecular dynamics simulations with enhanced sampling techniques to the bathy phytochrome and compared the results with those obtained for the canonical phytochrome .
View Article and Find Full Text PDFMicrob Biotechnol
June 2024
Forschungszentrum Jülich, Department for Plant Sciences (IBG-2), Institute of Bio- and Geosciences, Jülich, Germany.
Pathogens resistant to classical control strategies pose a significant threat to crop yield, with seeds being a major transmission route. Bacteriophages, viruses targeting bacteria, offer an environmentally sustainable biocontrol solution. In this study, we isolated and characterized two novel phages, Athelas and Alfirin, which infect Pseudomonas syringae and Agrobacterium fabrum, respectively, and included the recently published Pfeifenkraut phage infecting Xanthomonas translucens.
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