The mining and leakage of molybdenum (Mo) can cause environmental contamination which has not been realized until recently. Bacteria that can mitigate Mo-contamination was enriched and isolated. The low temperature and different pH conditions were considered to analysis its feasibility in Northern China which suffers from a long time of low temperatures every year. The result showed that the removal rate of MoO by Raoultella ornithinolytica A1 reached 30.46% at 25 °C and pH 7.0 in Luria-Bertani medium (LB). Meanwhile, A1 also showed some efficiency in the reduction of MoO in low phosphate molybdate medium (LPM), which reached optimum at the MoO concentration of 10 mM. The results of FTIR indicated that the cell wall performed an essential role in the MoO removal process, which was illustrated by the distribution of Mo in A1 (Mo bound to cell wall accounted for 92.29% of the total MoO removed). In addition, low temperature (10 °C) effect the removal rate of MoO by - 8.38 to 11.66%, indicating the potential for the in-situ microbial remediation of Mo-contaminated environments in low temperature areas.
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