A high-throughput SARS-CoV-2 pseudovirus multiplex neutralization assay.
STAR Protoc
J. Craig Venter Institute, La Jolla, CA 92037, USA; Division of Infectious Diseases, Department of Medicine, University of California San Diego, La Jolla, CA 92037, USA. Electronic address:
Published: December 2022
Evaluating the neutralizing antibody titer following SARS-CoV-2 vaccination is essential in defining correlates of protection. We describe an assay that uses single-cycle vesicular stomatitis virus (VSV) pseudoviruses linking a fluorophore with a spike (S) from a variant of concern (VOC). Using two fluorophores linked to two VOC S, respectively, allows us to determine the neutralization titer against two VOCs in a single run. This is a generalizable approach that saves time, samples, and run-to-run variability. For complete details on the use and execution of this protocol, please refer to Sievers et al. (2022)..
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9579189 | PMC |
| http://dx.doi.org/10.1016/j.xpro.2022.101835 | DOI Listing |
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