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Protocol to use de-epithelialized porcine urinary bladder as a tissue scaffold for propagation of pancreatic cells. | LitMetric

Protocol to use de-epithelialized porcine urinary bladder as a tissue scaffold for propagation of pancreatic cells.

STAR Protoc

Institute for Molecular Oncology and Stem Cell Biology, Ulm University Hospital, 89081 Ulm, Germany; Clinic of Internal Medicine I, Ulm University Hospital, 89081 Ulm, Germany; Core Facility Organoids, Ulm University, 89081 Ulm, Germany. Electronic address:

Published: December 2022

AI Article Synopsis

Article Abstract

Ex vivo organ culture can be a useful alternative to in vivo models, which can be time-, labor-, and cost-intensive. Here we describe a step-by-step protocol to use de-epithelialized porcine urinary bladders as scaffolds in air-liquid interface in vitro culture systems for a variety of pluripotent stem-cell-derived and patient-derived pancreatic cells and organoids. The scaffold can trigger cell maturation and enable cell-cell interaction and invasion capacity studies. However, this model is limited by the lack of functional vasculature. For complete details on the use and execution of this protocol, please refer to Melzer et al. (2022), Breunig et al. (2021), and Breunig et al. (2021)..

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9692066PMC
http://dx.doi.org/10.1016/j.xpro.2022.101869DOI Listing

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