Penicillenols belong to the family of tetramic acids with anticancer and antibacterial activities. Here, we report the discovery of the biosynthetic gene cluster ( for penicillenol A and E in ATCC9849 by genome mining. We discover the cluster based on the results of gene deletions in and gene cluster heterologous expression in . We also propose the assembly line of the PKS module in PncA with the reduction by PncB provides a highly reduce polyketide chain to be further linked with an L-threonine molecule and released from PncA to produce penicillenol E. Further formation of penicillenol A requires the -methylation of tetramic acid group by PncC. Our work deepens the understanding of the biosynthetic logic for -methylated tetramic acids and contributes to the discovery of new penicillenols by genome mining.
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| http://dx.doi.org/10.1002/aic.17885 | DOI Listing |
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