A PHP Error was encountered

Severity: Warning

Message: file_get_contents(https://...@pubfacts.com&api_key=b8daa3ad693db53b1410957c26c9a51b4908&a=1): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests

Filename: helpers/my_audit_helper.php

Line Number: 176

Backtrace:

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 176
Function: file_get_contents

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 250
Function: simplexml_load_file_from_url

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 3122
Function: getPubMedXML

File: /var/www/html/application/controllers/Detail.php
Line: 575
Function: pubMedSearch_Global

File: /var/www/html/application/controllers/Detail.php
Line: 489
Function: pubMedGetRelatedKeyword

File: /var/www/html/index.php
Line: 316
Function: require_once

A newly optimized protocol to extract high-quality hemolymph from the cattle tick : Improving the old conditions. | LitMetric

A newly optimized protocol to extract high-quality hemolymph from the cattle tick : Improving the old conditions.

Curr Res Parasitol Vector Borne Dis

Unidad de Artropodología del Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad, INIFAP, Carretera Federal Cuernavaca-Cuautla No. 8534, Progreso, 62550, Jiutepec, Morelos, Mexico.

Published: December 2021

Ticks are hematophagous ectoparasites with importance to animal and human health. In recent years, the study of ticks has had significant development, including immune response, vector-host interactions, physiological and multi-omics approaches. However, one of the main impediments is obtaining a significant amount of high-quality hemolymph. For this reason, we developed a protocol that allows obtaining up to 100 μl of hemolymph free of host blood per engorged tick. The technique consists of continuous hipocuticular punctures of the tick dorsum and an anticoagulant buffer that impedes hemolymph coagulation, allowing constant extravasation and ensuring high yields. Additionally, the hemocytes recovered with this protocol are intact and can be used for further analysis. The high-quality hemolymph obtained using this protocol and its applications will help to better understand the processes involving the hemolymph and its components. Although there are other hemolymph extraction protocols, the method developed here is very well suited for , and in our experience, results in better yields and high-quality samples.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9795341PMC
http://dx.doi.org/10.1016/j.crpvbd.2021.100066DOI Listing

Publication Analysis

Top Keywords

high-quality hemolymph
12
hemolymph
7
newly optimized
4
protocol
4
optimized protocol
4
protocol extract
4
high-quality
4
extract high-quality
4
hemolymph cattle
4
cattle tick
4

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!