AI Article Synopsis

  • Cystathionine beta synthase (CBS) domains containing proteins (CDCPs) are crucial for plant development and stress responses, but a comprehensive study on wheat's CBS gene family and its connection to high-temperature male sterility is lacking.
  • In this research, 66 CBS family members were identified in the wheat genome, categorized into four subgroups, and their distribution was found to be uneven across the 21 chromosomes.
  • Analysis revealed significant associations of CBS genes with pollination and reproductive processes, along with evidence that certain genes play a critical role in anther development under heat stress, suggesting a link between CBS genes and male sterility in wheat.

Article Abstract

Cystathionine beta synthase (CBS) domains containing proteins (CDCPs) plays an important role in plant development through regulation of the thioredoxin system, as well as its ability to respond to biotic and abiotic stress conditions. Despite this, no systematic study has examined the wheat CBS gene family and its relation to high temperature-induced male sterility. In this study, 66 CBS family members were identified in the wheat genome, and their gene or protein sequences were used for subsequent analysis. The gene family was found to be unevenly distributed on 21 chromosomes, and they were classified into four subgroups according to their gene structure and phylogeny. The results of collinearity analysis showed that there were 25 shared orthologous genes between wheat, rice and , and one shared orthologous gene between wheat, millet and barley. The cis-regulatory elements of the were related to JA, IAA, MYB, etc. GO and KEGG pathway analysis identified these genes to be associated with pollination, reproduction, and signaling and cellular processes, respectively. A heatmap of wheat plants based on transcriptome data showed that genes were expressed to a higher extent in spikelets relative to other tissues. In addition, 29 putative tae-miRNAs were identified, targeting 41 genes. Moreover, qRT-PCR validation of six genes indicated their critical role in anther development, as five of them were expressed at lower levels in heat-stressed male sterile anthers than in Normal anthers. Together with anther phenotypes, paraffin sections, starch potassium iodide staining, and qRT-PCR data, we hypothesized that the gene has a very important connection with the heat-stressed sterility process in wheat, and these data provide a basis for further insight into their relationship.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9795209PMC
http://dx.doi.org/10.3389/fpls.2022.1061472DOI Listing

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