Lakes with or without urbanization along their coasts had similar level of microplastic contamination, but significant differences were seen between sampling methods.

The influence of sampling method on microplastic (MP) quantification and the impact of population density on the levels of MP contamination in surface waters from Patagonian lakes were investigated. Six lakes located in Northern Patagonia (Argentina) were studied using two different sampling protocols widely reported in the literature: water collected in glass bottles vs. water collected using a 50 μm mesh size net. To assess the influence of population density on MP contamination, lakes with urbanization at shores (Nahuel Huapi, Gutierrez and Moreno) and lakes without urbanization on their shores (Espejo, Espejo Chico and Mascardi) were considered. We identified contamination with secondary MP at all the freshwater lakes studied, with predominance (>90 %) of textile-based microfibers (MF). Remarkably the levels of contamination were similar in all the lakes, independently of whether they were impacted by urbanization along their coasts or not, which supports the notion that there is atmospheric transport of MP. The greatest variability found was among sampling methods, with differences above of three orders magnitude. Samples collected directly in 1-l bottles had an average of 5257 MP/m in comparison to 1.57 MP/m in the samples that were collected with a 50 μm net. Interestingly, Nahuel Huapi lake samples collected with bottles where the WWTP discharges effluents were significantly more contaminated (SD 9400 ± 4351 MP/MF per m) than samples collected 5 km west of the plant (2100 ± 1197 MP/MF per m). Results highlight the significance of textile microfibers as microplastic contaminants of freshwater, and the need to address mesh size when looking for textile microfibers and to develop standardized sampling protocols to make studies on freshwater MF contamination comparable.