Polyhydroxyalkanoate (PHA) production has been the focus of considerable research to increase productivities and reduce production costs. In this study, a fermented confectionary industry wastewater was used as feedstock for mixed microbial culture PHA production. The feedstock was dominated by lactate and ethanol (60-90 % of all soluble fermentation products). The culture selection reactor was inoculated with municipal activated sludge and was operated at an organic loading rate (OLR) of 100 Cmmol·L·d, achieving a robust PHA-accumulating enrichment, which produced up to 52.6 ± 0.4 wt% of PHA in accumulation assays. An OLR increase in the culture selection stage to 150 Cmmol·L·d led to a PHA content of 59.1 ± 0.6, a yield of 0.93 ± 0.01 Cmol-PHA·Cmol-S and a productivity of 0.93 ± 0.01 g-PHA L·h. A correlation analysis of the impact of ethanol concentrations from 3.19 to 20.3 Cmmol·L in the reactor showed that ethanol inhibited PHA production rate and yield and the consumption of other carbon sources available. Microbial community analysis revealed the increase of Amaricoccus genus during the bioreactor operation time, a known PHA accumulator. The produced polymer was poly(3-hydroxybutyrate) with an average molecular weight of 4.3 × 10 Da and a polydispersity index of 1.88.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.ijbiomac.2022.12.268 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Assessing the efficiency and potential for internally reusing nitrogen-containing effluent in the PHA accumulation stage under low C/N conditions in a mixed-culture process.
Bioresour Technol
December 2024
School of Water Conservancy and Transportation, Zhengzhou University, Zhengzhou 450001, China. Electronic address:
Article Synopsis
- Polyhydroxyalkanoates (PHAs) are biodegradable plastics that can be produced through a mixed culture-based process, but ammonia nitrogen can hinder this production.
- This study explores ways to efficiently reuse ammonia nitrogen to enhance PHA synthesis and reduce waste.
- Results showed a significant increase in PHA production when using specific substrate and process conditions, while also effectively recycling ammonia without negatively affecting the mixed culture's properties.
Lignocellulosic biomass as promising substrate for polyhydroxyalkanoate production: Advances and perspectives.
Biotechnol Adv
December 2024
State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, PR China. Electronic address:
The depletion of fossil resources, coupled with global warming and adverse environmental impact of traditional petroleum-based plastics, have necessitated the discovery of renewable resources and innovative biodegradable materials. Lignocellulosic biomass (LB) emerges as a highly promising, sustainable and eco-friendly approach for accumulating polyhydroxyalkanoate (PHA), as it completely bypasses the problem of "competition for food". This sustainable and economically efficient feedstock has the potential to lower PHA production costs and facilitate its competitive commercialization, and support the principles of circular bioeconomy.
View Article and Find Full Text PDFProteomic analysis of natural photoheterotrophic mixed consortium for biohydrogen production under nongrowing conditions.
Bioresour Technol
December 2024
Bioprocesses Department, Instituto Politécnico Nacional, P.O. Box 07340, Mexico City, Mexico. Electronic address:
A biohydrogen and polyhydroxyalkanoates (PHA)-producing natural photoheterotrophic mixed culture composed mainly by Rhodopseudomonas palustris and Clostridium sp was studied by a proteomic analysis under non-growth conditions (nitrogen-absence and organic acids). Proteins in C. pasteurianum were upregulated, particularly those related to stress response.
View Article and Find Full Text PDFConverting multiple hydrophobic aromatic plastic monomers into a single water-soluble substrate to increase bioavailability for the synthesis of polyhydroxyalkanoates by bacteria using batch, fed batch and continuous cultivation.
J Biotechnol
December 2024
School of Biomolecular and Biomedical Sciences, University College Dublin, Dublin D04 N2E5, Ireland; BiOrbic Bioeconomy Research Centre, O'Brien Centre for Science [Science East], University College Dublin, Dublin D04 N2E5, Ireland. Electronic address:
We demonstrate the proof of concept of increasing the bioavailability of carbon substrates, derived from plastic waste, for their conversion to the biodegradable polymer polyhydroxyalkanoate [PHA] by bacteria and test various approaches to PHA accumulation through batch, fed batch and continuous culture. Styrene, ethylbenzene, and toluene are produced from the pyrolysis of mixed plastic waste (Kaminsky, 2021; Miandad et al., 2017), but they are volatile and poorly soluble in water making them difficult to work with in aqueous fermentation systems.
View Article and Find Full Text PDFAir-Mediated Biomimetic Synthesis of Polyhydroxyalkanoate with C4 Diol.
Angew Chem Int Ed Engl
December 2024
Chemistry and Chemical Engineering Guangdong Laboratory, 515031, Shantou, China.
Article Synopsis
- Poly(4-hydroxybutyrate) (P4HB) is a biodegradable polymer traditionally made through complex and costly genetic engineering processes involving 1,4-butanediol (BDO).
- A new catalytic method using a ruthenium-phosphine system for the oxidation of BDO has shown improved efficiency and cost-effectiveness, converting BDO into valuable products like γ-butyrolactone and oligomeric P4HB.
- This approach not only enhances production yield but also offers the potential for sustainable and large-scale manufacturing of high-value polyhydroxyalkanoates (PHAs) from non-grain feedstocks.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!