The ligand-receptor complexes in the cell-cell and cell-ECM interfaces mediate the mechanical coupling of cells to their microenvironment and transduce downstream signals to modulate cell functions. In this chapter I describe a microfabrication strategy to prepare a substrate of spatially segregated supported lipid bilayers and ECM components on which cells can form juxtacrine receptor signaling complexes and integrin adhesions simultaneously. This platform is specifically applicable for microscopically monitoring the signal transduction of each receptor, as well as the modulatory effects of receptor signaling on integrin adhesions and cell behaviors.
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