AI Article Synopsis
- - The study investigates how the stiffness of soil affects the growth and cell development of plant roots and root hair cells, an important research area in plant biology.
- - A new method called the microfluidic-like system (MLS) is introduced, allowing researchers to grow plantlets in gels of varying stiffness over extended periods while observing them via microscopy.
- - MLS combines benefits of microfluidic technology such as precise control over growth conditions and high-resolution imaging, facilitating long-term quantitative research on how environmental rigidity influences root growth and cellular responses.
Article Abstract
Plant's roots grow in soils of different rigidities. Understanding how the stiffness of the surrounding environment impacts growth and cell fate of roots and root hair cells is an important and open question. Here, we describe a simple method to setup a microfluidic-like system (MLS) to tackle this question. This system enables to grow plantlets during weeks in microfluidic chips filled with gels of controlled stiffness and to image them under a microscope from a few minutes up to a few days. Furthermore, MLS keeps the numerous benefits of microfluidic chips, such as high-resolution imaging, precise control of the geometry of growth, and standardization of the measurements. In sum, MLS enables one to quantitatively test, even on long time scales, the effect of the rigidity and the geometry of the environment on the growth of roots and root hair cells, including mechanotransduction to the nucleus.
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| http://dx.doi.org/10.1007/978-1-0716-2851-5_8 | DOI Listing |
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