The evolutionary journey of freshwater crabs of the genus Potamon (Decapoda: Brachyura: Potamidae).

Aim: Freshwater ecosystems host a rich biodiversity, including freshwater crabs. The family Potamidae is a diverse group of freshwater crabs with a Palearctic and Oriental distribution. Specifically, the genus Potamon is found in the Middle East, Southern Europe, and Northern Africa. Potamon species are considered true freshwater crabs due to their total independence from the marine environment, which makes them idea organisms to study historical freshwater connectivity dynamics. In this study we aim to elucidate the phylogenetic relationships of the genus Potamon and discuss the historical freshwater connectivity in the Mediterranean region.

Methods: Here we use up to eleven genetic markers to reconstruct a robust phylogenetic tree of the group and use Bayesian methods to time-calibrate the tree. We further use historical biogeography methods implemented in a Bayesian framework to assess the ancestral distribution ranges of the group.

Results: In this study we generate the most complete dataset for the group, covering practically the whole distribution range of the genus. We obtained a robust phylogenetic hypothesis and evaluated the times of divergence of the group. The result of the historical biogeography shows the evolution of historical distribution ranges of species of Potamon.

Main Conclusions: The Mediterranean Sea is placed in a key intersection for the exchange of fauna and flora. Here we present evidence for the potential of freshwater fauna exchange, through the Mediterranean Sea after a period of desiccation and the loss of contact with the ocean. The origin of Potamon genus is found to be in Western Asia, probably Eastern Iran.