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Combining IP affinity chromatography and bioinformatics reveals a novel protein-IP binding site on MDR1 transporter. | LitMetric
Intracellular Ca mobilization induced by second messenger IP controls many cellular events in most of the eukaryotic groups. Despite the increasing evidence of IP-induced Ca in apicomplexan parasites like , responsible for malaria infection, no protein with potential function as an IP-receptor has been identified. The use of bioinformatic analyses based on previously known sequences of IP-receptor failed to identify potential IP-receptor candidates in any . In this work, we combine the biochemical approach of an IP affinity chromatography column with bioinformatic meta-analyses to identify potential vital membrane proteins that present binding with IP in . Our analyses reveal that PF3D7_0523000, a gene that codes a transport protein associated with multidrug resistance as a potential target for IP. This work provides a new insight for probing potential candidates for IP-receptor in .
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