Objective: Acute kidney injury (AKI) is one of common complications of wasp/bee stings. Phospholipase A (PLA) is a vital pathogenic composition of wasp/bee venom. We aimed to investigate the role of complement mediated mitochondrial apoptosis in PLA induced AKI.
Materials And Methods: PLA induced AKI model was established by injecting PLA into via tail vein on mice. The pathological changes and the microstructural changes of kidney, complement activation, inflammation and apoptosis were detected in vitro and in vivo respectively.
Results: The results showed that PLA induced AKI models were successfully established in vivo and vitro. Compared with control, serum creatinine and urea nitrogen levels were elevated. Complement system activation and mitochondrial damage were observed. Expressions of IL-6, TNF-α, cleaved caspase-3 and cleaved caspase-9, and Bax/Bcl-2 increased in PLA induced AKI models. TNF-α/NF-κB signaling pathway activation in AKI models.
Conclusion: In the present study, PLA induced AKI model was first successfully established to our knowledge. The role of complement mediated mitochondrial apoptosis pathway in renal tubular epithelial cells in PLA induced AKI were verified in vitro and vivo.
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