Circular RNAs (circRNAs) are a form of RNAs that lack coding potential. The role of such circRNAs in dental pulp stem cell (DPSC) osteo/odontogenic differentiation remains to be determined. In this study, circRNA expression profiles in DPSC osteo/odontogenic differentiation process were analyzed by RNA-seq. qRT-PCR was used to confirm the differential expression of , , and in DPSC osteogenic differentiation process. , , and were knocked down or overexpressed. Osteoblastic activity and associated mineral activity were monitored via alkaline phosphatase () and alizarin red S (ARS) staining. Interactions between , , and were assessed through luciferase reporter assays. Finally, an system was used to confirm the relevance of to osteoblastic differentiation. As results, we detected significant and upregulation in DPSC osteo/odontogenic differentiation process, as well as concomitant downregulation. When knocking down or overexpressed , this significantly inhibited osteogenesis. Luciferase reporter assay confirmed that was capable of binding to conserved sequences in the wild-type forms of both the and . Furthermore, knocking down significantly attenuated bone formation. Therefore, the axis regulates DPSC osteo/odontogenic differentiation, which may provide potential molecular targets for dental-pulp complex regeneration.
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