We present a protocol to detect extracellular traps (ETs) induced by Cutibacterium acnes in cultured T17 clones. We first describe the isolation of C. acnes-specific T17 clones by sterile cell sorting. We then detail the in vitro induction of ETs in T17 clones stimulated by C. acnes and the imaging of released ETs using scanning electron microscopy. This protocol can be applied to the study of other ETs released by other T cell subsets. For complete details on the use and execution of this protocol, please refer to Agak et al. (2021)..
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9813782 | PMC |
| http://dx.doi.org/10.1016/j.xpro.2022.101932 | DOI Listing |
Publication Analysis
Top Keywords
t17 clones
12
protocol detect
8
extracellular traps
8
scanning electron
8
electron microscopy
8
microscopy protocol
8
protocol
4
detect human
4
human cd4
4
cd4 t cell
4
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!