Evaluating the value of anti-SARS-CoV-2 antibody detection and neutralizing responses with euvirus: A population of 10776 close contacts in the epidemic of Fujian.

Clin Chim Acta

Department of Laboratory Medicine, Gene Diagnosis Research Center, The First Affiliated Hospital, Fujian Medical University, Fuzhou, PR China; Fujian Key Laboratory of Laboratory Medicine, The First Affiliated Hospital, Fujian Medical University, Fuzhou, PR China. Electronic address:

Published: January 2023

AI Article Synopsis

  • Nucleic acid detection for COVID-19 has limitations like false negatives; thus, anti-SARS-CoV-2 antibody tests have become widely utilized, but their effectiveness in China had not been thoroughly studied.
  • Researchers collected 10,776 serum samples from close contacts of COVID-19 cases in Fujian, China, using both chemiluminescence and lateral flow immunoassays to conduct antibody testing.
  • Results showed that the antibody tests had high diagnostic accuracy, especially in neutralizing antibodies, with strong correlation found between antibody detection methods and neutralization assays, indicating their significant value in diagnosing COVID-19.

Article Abstract

Background: Nucleic acid detection represents limitations due to its false-negative rate and technical complexity in the COVID-19 pandemic. Anti-severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibody tests are widely spread all over the world presently. However, there is no report on the effectiveness of anti-SARS-CoV-2 antibody testing methods in China.

Methods: We gathered 10776 serum samples from close contacts of the SARS-CoV-2 infections in Fujian of China and used 2 chemiluminescence immunoassays (Wantai Bio., Yahuilong Bio.) and 2 lateral flow immunoassays (Lizhu Bio. and Dongfang Bio.) to perform the anti-SARS-CoV-2 antibody tests in China.

Results: The 4 antibody tests have great diagnostic value for infected or uninfected, especially in the neutralizing antibodies tests, the AUC can reach 0.939 (Wantai Bio.) and 0.916 (Yahuilong Bio.). Furthermore, we used pseudoviruses and euvirus neutralization assay to validate the effectiveness of these antibody test, the results of pseudoviruses neutralization assay or euvirus neutralization assay shows a considerable correlation with the 4 antibody detection respectively, particularly in euvirus neutralization assay, neutralizing antibodies detected by Wantai Bio. or Yahuilong Bio., the correlation can get the level of 0.93 or 0.82.

Conclusions: The findings of this study demonstrate that the detections of antibodies have profound value in the diagnosis of COVID-19.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9783188PMC
http://dx.doi.org/10.1016/j.cca.2022.12.017DOI Listing

Publication Analysis

Top Keywords

neutralization assay
16
anti-sars-cov-2 antibody
12
antibody tests
12
wantai bio
12
yahuilong bio
12
euvirus neutralization
12
antibody detection
8
close contacts
8
bio
8
bio yahuilong
8

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!