Objective: The aim of the current study was to investigate the contributing role of gene variation and transcription levels among the m6A methyltransferases METTL3, METTL14, and WTAP in pulmonary tuberculosis (PTB).
Methods: A case-control study including 461 PTB patients and 467 normal controls was designed for genotyping. Three SNPs in (rs1061027, rs1139130, rs1061026), three SNPs in (rs62328061, rs4834698, rs1064034), and two SNPs in (rs1853259, rs11752345) were genotyped the SNPscan™ technique. , , and transcription levels were determined in 78 PTB patients and 86 controls quantitative real-time reverse-transcription PCR.
Results: Frequencies of the rs62328061 GG genotype, rs11752345 CT genotype, and T allele were significantly increased in PTB patients compared to controls. An increased risk of rs62328061 was detected in a recessive model, and a decreased risk of rs11752345 was detected in a dominant model in the PTB group. gene variation was not associated with PTB risk. The rs1139130 GG genotype was significantly increased with drug resistance, and the G allele was significantly decreased with drug-induced liver injury in PTB patients. A reduced frequency of the rs62328061 G allele was associated with leukopenia, a reduced frequency of the rs11752345 T allele was associated with sputum smear positivity, and a higher frequency of the rs4834698 TC genotype was evident in PTB patients with hypoproteinemia. Compared to controls, , , and transcription levels in PTB patients were significantly decreased, and the level of WTAP was increased in PTB patients with drug resistance. METTL3 level was negatively associated with erythrocyte sedimentation rate and aspartate aminotransferase, and METTL14 level was negatively correlated with alanine aminotransferase and aspartate aminotransferase.
Conclusion: rs62328061 and rs11752345 variants were associated with the genetic background of PTB, and METTL3, METTL14, and WTAP levels were abnormally decreased, suggesting that these m6A methyltransferases may play important roles in PTB.
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http://dx.doi.org/10.3389/fimmu.2022.992628 | DOI Listing |
Front Nutr
December 2024
Department of Infectious Diseases, The Ninth People's Hospital of Chongqing, Chongqing, China.
Background: Although malnutrition is associated with poor prognosis in Pulmonary Tuberculosis (PTB) patients, no nutrition-based prediction model has been established for PTB. Herein, we explored the clinical utility of common nutrition scores in predicting the prognosis of PTB patients.
Methods: We retrospectively collected clinical baseline data from 167 patients with secondary PTB who had not previously received anti-TB treatment.
Gene
December 2024
Scientific Research Center, The Third Affiliated Hospital of Zunyi Medical University (The First People's Hospital of Zunyi), Zunyi, Guizhou, China; Department of Clinical Laboratory, The Third Affiliated Hospital of Zunyi Medical University (The First People's Hospital of Zunyi), Zunyi, Guizhou, China. Electronic address:
Pre-existing of pulmonary tuberculosis (PTB) poses increased lung cancer risk, yet the molecular mechanisms remain inadequately understood. This study sought to elucidate the potential mechanisms by performing comprehensive analyses of differentially expressed genes (DEGs) in peripheral blood mononuclear cells (PBMCs) from patients with PTB, lung adenocarcinoma (LUAD), and lung squamous cell carcinoma (LUSC). Microarray assays were employed to analyze the DEGs in PBMCs of these patients.
View Article and Find Full Text PDFSyst Rev
December 2024
Department of Respiratory and Critical Care Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Background: Metagenomic next-generation sequencing (mNGS) has emerged as a promising tool in clinical practice due to its unbiased approach to pathogen detection. Its diagnostic performance in pulmonary tuberculosis (PTB), however, remains to be fully evaluated.
Objective: This study aims to systematically review and Meta-analyze the diagnostic accuracy of mNGS in patients with PTB.
BMC Infect Dis
December 2024
Department of Epidemiology and Biostatistics, School of Public Health, Isfahan University of Medical Sciences, Isfahan, Iran.
Background: Worldwide, tuberculosis (TB) is among the most common causes of death. To our knowledge, there has been no study showing the prevalence of EPTB in Khuzestan province. Therefore, the objective of this research was to investigate the prevalence of EPTB in patients with or without pulmonary TB in different cities of Khuzestan province from 2002 to 2023.
View Article and Find Full Text PDFSci Rep
December 2024
Department of Medical Application of Laser, Ophthalmic Unit, National Institute of Laser Enhanced Sciences, Cairo University, Giza, Egypt.
Corneal alkali burns persist as a significant challenge in our field, often leading to a prolonged treatment course with various sight-threatening problems. This work, of utmost importance, aimed to apply the photo-tissue bonding technique (PTB) to weld the amniotic membrane (AM) to the corneal surface versus an amniotic membrane graft (AMG) and explore its safety in saving corneal protein against alkali burn.Methods Twenty-seven rabbits with an induced corneal ulcer using 1 mol/L NaOH solution.
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