Expression of heterologous heparan sulphate binding protein of on the surface of GG.

Unlabelled: () is one of most commonly found pathogen in the stomach. In spite of emergence of different treatment strategies, infection remains difficult to treat. The bioengineered probiotic lactobacilli that could displace and simultaneously present immunogenic peptides such as heparan sulphate binding protein (Hsbp) to elicit immune response could emerge as a potential therapeutic agent. The aim of this study was to discover the anti- activities and faster exclusion of from host cells by the recombinant strain of expressing the immunogenic Hsbp protein. The results were promising and showed a 65% reduction in adhesion after two hours of pre-incubation with recombinant-LGG and HeLa S3 cells, followed by the adhesion of pathogen ( < 0.002). Additionally, 36% and 39% reduction were examined in co-incubation and post-incubation with recombinant-LGG, respectively. When challenged with , the proinflammatory cytokine expression was also down regulated in recombinant-LGG treated HeLa S3 cells. This promising result provides a new insight of bioengineered probiotic lactobacilli which could displace and simultaneously has immunogenic properties thereby may be useful to prevent infection.

Supplementary Information: The online version contains supplementary material available at 10.1007/s13205-022-03428-4.