AI Article Synopsis
- Dynein light chain (DLC-1) is crucial for P granule integrity in embryos and acts as a regulator in multi-subunit protein complexes.
- P granules are essential for RNA regulation and fertility, composed of RNA-protein complexes found in the germline.
- A study using mass spectrometry showed that DLC-1 knockdown did not affect core P granule or nucleoporin protein levels, suggesting its role in facilitating phase separation rather than regulating protein levels.
Article Abstract
Dynein light chain (DLC-1) is a light chain component of the dynein motor complex, it functions as an allosteric regulator of multi-subunit protein complexes and promotes P granule integrity in the embryo. P granules are RNA-protein complexes located in the germline that are important for RNA regulation and fertility. To further study the role of DLC-1 during embryogenesis we performed quantitative tandem mass tag mass spectrometry on embryos after knock down. The amount of core P granule components and nucleoporin proteins did not change after These results show that DLC-1 does not help regulate P granule protein levels and support the model that DLC-1 facilitates phase separation of P granule components .
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Source |
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9772925 | PMC |
| http://dx.doi.org/10.17912/micropub.biology.000700 | DOI Listing |
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