Clean feed as countermeasure to reduce the Sr and Cs levels in fish from contaminated lakes.

Glubokoye Lake situated within the Chernobyl Exclusion Zone is highly contaminated with respect to radioactive caesium and strontium isotopes, which also is reflected in the contaminated fish. To utilize the fish resources in contaminated lakes, the present work presents for the first time the effectiveness of using clean feed to counteract contamination of radionuclides in fish. The study is based on a series of repeated experiments with Prussian carp (Carassius gibelio (Bloch, 1782)) kept in cages in the contaminated Glubokoye Lake during summer 2018-2021. By the addition of clean feed, the activity concentration of Cs in fish muscle tissues was lowered with a factor of 2-5 due to biodilution. Surprisingly, additional clean feed did not lead to further decrease in the uptake of Cs in fish. In contrast to Cs, the addition of clean feed increased the Sr activity concentration in fish by a factor of 2-4 compared to fish fed with naturally occurring feed items. Radioactive strontium accumulated mainly in the fish bones and the muscle tissue level was 2 orders of magnitude lower, similar to the distribution observed for stable Sr. By utilizing a new kinetic model describing the dynamics of strontium isotopes in bone tissues of fish, predictions fitted well with site-specific data, taking growth rates and aging into account. Results showed that clean feeding can be used to counteract high activity concentration of Cs in fish due to biodilution, but cannot counteract bioaccumulation of Sr. Findings highlighted that it is essential to understand underlying factors influencing the uptake pathways for contaminants, as access to clean feed could increase the growth and thereby reduce the body activity concentration of dietary associated radionuclides such as Cs (biodilution), as well as increase the transfer of dissolved compounds such as Sr directly from water to fish.