AI Article Synopsis
- * In this study, MUS reduced fat accumulation and increased fat breakdown (lipolysis) in mature 3T3-L1 cells, while also promoting cell death (apoptosis) and lowering the levels of proteins that promote fat storage.
- * The research suggests that MUS acts through a specific pathway (PKA/p38) to help decrease fat buildup in cells, indicating its potential as a low-side-effect treatment option for obesity.
Article Abstract
Musclin (MUS), an exercise-responsive myokine, has been documented to attenuate inflammation and enhance physical endurance. However, the effects of MUS on differentiation and related molecular mechanisms in adipocytes have not yet been studied. In this study, we found that treatment with MUS attenuated lipid accumulation in fully differentiated 3T3-L1 cells. Furthermore, MUS treatment enhanced lipolysis assessed by glycerol release, and caused apoptosis, whereas it reduced the expression of lipogenic proteins, such as PPARγ and processed SREBP1. Treatment with MUS augmented phosphorylated PKA expression, whereas suppressed p38 phosphorylation in 3T3-L1 adipocytes. H89, a selective PKA inhibitor reduced the effects of MUS on lipogenic lipid accumulation as well as lipolysis except for apoptosis. These results suggest that MUS promotes lipolysis and suppresses lipogenesis through a PKA/p38-dependent pathway, thereby ameliorating lipid deposition in cultured adipocytes. The current study offers the potential of MUS as a therapeutic approach for treating obesity with few side effects.
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| http://dx.doi.org/10.1016/j.bbrc.2022.12.056 | DOI Listing |
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