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Potential of [C]()-PK11195 PET Imaging for Evaluating Tumor Inflammation: A Murine Mammary Tumor Model. | LitMetric

Potential of [C]()-PK11195 PET Imaging for Evaluating Tumor Inflammation: A Murine Mammary Tumor Model.

Pharmaceutics

Laboratory of Nuclear Medicine (LIM 43), Department of Radiology and Oncology, Faculdade de Medicina FMUSP, Universidade de Sao Paulo, Sao Paulo 05403-911, Brazil.

Published: December 2022

AI Article Synopsis

Article Abstract

Background: Breast tumor inflammation is an immunological process that occurs mainly by mediation of Tumor-Associated Macrophages (TAM). Aiming for a specific measurement of tumor inflammation, the current study evaluated the potential of Positron Emission Tomography (PET) imaging with [C]()-PK11195 to evaluate tumor inflammation in a mammary tumor animal model.

Methods: Female Balb/C mice were inoculated with 4T1 cells. The PET imaging with [C]()-PK11195 and [F]FDG was acquired 3 days, 1 week, and 2 weeks after cell inoculation.

Results: The [C]()-PK11195 tumor uptake increased from 3 days to 1 week, and decreased at 2 weeks after cell inoculation, as opposed to the [F]FDG uptake, which showed a slight decrease in uptake at 1 week and increased uptake at 2 weeks. In the control group, no significant differences occurred in tracer uptake over time. Tumor uptake of both radiopharmaceuticals is more expressed in tumor edge regions, with greater intensity at 2 weeks, as demonstrated by [C]()-PK11195 autoradiography and immunofluorescence with TSPO antibodies and CD86 pro-inflammatory phenotype.

Conclusion: The [C]()-PK11195 was able to identify heterogeneous tumor inflammation in a murine model of breast cancer and the uptake varied according to tumor size. Together with the glycolytic marker [F]FDG, molecular imaging with [C]()-PK11195 may provide a better characterization of inflammatory responses in cancer.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9786563PMC
http://dx.doi.org/10.3390/pharmaceutics14122715DOI Listing

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