Background: is an important multidrug-resistant (MDR) pathogen, causing both community- and healthcare-associated infections. The resistance is due to the continuous accumulation of multiple antibiotic-resistance-genes (ARGs) through spontaneous genomic mutations and the acquisition of conjugative plasmids. This study presents antibiotics resistance genes, plasmids replicons, and virulence genes of isolates from clinical specimens in a tertiary hospital, Mwanza, Tanzania.
Methods: Whole genome sequencing (WGS) of 34 was performed, using an Illumina NextSeq 500, followed by in silco analysis.
Results: A total of 34 extended-spectrum beta-lactamase-producing , isolated from blood samples from neonatal units were whole-genome sequenced. Of these, 28 (82.4%) had an identified sequence type (ST), with ST14 (39.3%, n = 11) being frequently identified. Moreover, 18 (52.9%) of the bacteria harbored at least one plasmid, from which a total of 25 plasmid replicons were identified with a predominance of IncFIB(K) 48.0% (n = 12). Out of 34 sequenced , 32 (94.1%) were harboring acquired antibiotic/biocides-resistance-genes (ARGs) with a predominance of (90.6%), followed by B (87.5%), A (84.4%), (84.4%) and 2 (84.4%). Interestingly, we observed the ColRNAI plasmid-replicon (n = 1) and E gene (n = 4) for the first time in this setting.
Conclusion: Global high-risk clones of isolates carry multiple ARGs in multiple plasmid-replicons. Findings from this study warrant genomic-based surveillance to monitor high-risk global clones, epidemic plasmids and ARGs in low- and middle-income countries.
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| http://dx.doi.org/10.3390/microorganisms10122396 | DOI Listing |
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