Background: Stemness markers play critical roles in the maintenance of key properties of embryonic stem cells (ESCs), including the pluripotency, stemness state, and self-renewal capacities, as well as cell fate decision. Some of these features are present in cancer stem cells (CSCs). TWIST1, as a bHLH transcription factor oncogene, is involved in the epithelial-mesenchymal transition (EMT) process in both embryonic and cancer development. Our aim in this study was to investigate the functional correlation between and the involved genes in the process of CSCs self-renewal in human esophageal squamous cell carcinoma (ESCC) line KYSE-30.
Methods: overexpression was enforced in the ESCC KYSE-30 cells using retroviral vector containing the specific pruf-IRES-GFP-hTWIST1 sequence. Following RNA extraction and cDNA synthesis, the mRNA expression profile of and the stem cell markers, including , , , , , and were assessed using relative comparative real-time PCR.
Results: Ectopic expression of in KYSE-30 cells resulted in an increased expression of compared to control GFP cells by nearly 9-fold. Transduction of -retroviral particles caused a significant enhancement in , , , and mRNA expression, approximately 4.5-, 3.2-, 5.5-, 3.5-, and 3.7-folds, respectively, whereas this increased expression caused no change in the mRNA expression of and genes.
Conclusions: gene ectopic expression in KYSE-30 cells enhanced the level of cancer stem cell markers' mRNA expression. These results may emphasize the role of in the self-renewal process and may corroborate the involvement of in the stemness state capacity of ESCC cell line KYSE-30, as well as its potential as a therapeutic target.
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| http://dx.doi.org/10.3390/genes13122369 | DOI Listing |
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