Characterization of Isolated from Raw Milk.

is an opportunistic pathogen associated with nosocomial infections. In this study, 100 raw milk samples were collected from Qena, Egypt, and subjected to conventional and molecular assays to determine the presence of and investigate their antimicrobial resistance and biofilm formation. Our findings revealed that, among the 100 samples, spp. were found in 13 samples based on CHROM agar results. We further characterized them using B and sequencing and B multiplex PCR analysis and confirmed that 9 out of the 13 spp. isolates were and 4 were other species. The isolates were resistant to β-lactam drugs, including cefotaxime (44%), ampicillin-sulbactam and levofloxacin (33.3% for each), imipenem, meropenem and aztreonam (22.2% for each). We observed different antimicrobial resistance patterns, with a multi-antibiotic resistant (MAR) index ranging from 0.2 to 0.3. According to the PCR results, and genes were amplified in 100% and 55.5% of the isolates, respectively, while the gene was not amplified. Furthermore, the metallo-β-lactamases (MBL) genes and were found in 11.1% and 22.2% of isolates, respectively, while was not amplified. Additionally, eight isolates (88.8%) produced black-colored colonies on Congo red agar, demonstrating their biofilm production capacity. These results showed that, besides other foodborne pathogens, raw milk should also be examined for , which could be a public health concern.