AI Article Synopsis
- Phthalates (PAEs) are common pollutants found in soil and food, posing serious health risks to people.
- Scientists identified a diverse range of bacteria capable of breaking down PAEs, particularly focusing on a strain called Raoultella ornithinolytica XF201, which was isolated from wild rice.
- By using ribosome engineering, researchers activated specific genes in a mutant version of this strain (XF201-G2U5), enabling it to significantly degrade di-n butyl phthalate (DBP) and other related contaminants, demonstrating potential for bioremediation efforts.
Article Abstract
Phthalates (PAEs) are ubiquitous in soils and food products and thus pose a high risk to human health. Herein, genome mining revealed a great diversity of bacteria with PAEs-degrading potential. Mining of the genome of Raoultella ornithinolytica XF201, a novel strain isolated from Dongxiang wild rice rhizosphere, revealed the presence of two silenced tandem genes pcdGH (encoding protocatechuate 3,4-dioxygenase, 3,4-PCD), key aromatic ring-cleaving genes in PAEs biodegradation. Ribosome engineering was successfully utilized to activate the expression of pcdGH genes to produce 3,4-PCD in the mutant XF201-G2U5. The mutant XF201-G2U5 showed high 3,4-PCD activity and could remove 94.5 % of di-n butyl phthalate (DBP) in 72 h. The degradation kinetics obeyed the first-order kinetic model. Strain XF201-G2U5 could also degrade the other PAEs and the main intermediate metabolites, ultimately leading to tricarboxylic acid cycle. Therefore, this strategy facilitates novel bacterial resources discovery for bioremediation of PAEs and other emerging contaminants.
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| http://dx.doi.org/10.1016/j.scitotenv.2022.161013 | DOI Listing |
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