AI Article Synopsis
- - The study aimed to compare the microbiota of cholesteatomas and chronic suppurative otitis media (COM) to identify potential pathogens linked to cholesteatoma phenotypes.
- - Analysis of surgical specimens showed significant differences in bacterial composition between cholesteatomas and COM, with certain bacteria being more prevalent in cholesteatomas.
- - Findings indicate that coagulase-negative staphylococci (CoNS) could serve as biomarkers for cholesteatomas, and their abundance was positively correlated with the size of the cholesteatoma, suggesting their role in disease progression.
Article Abstract
Objective: To compare the microbiota between cholesteatoma and chronic suppurative otitis media (COM) and to identify potential pathogens that explain the relevant phenotypes of cholesteatoma.
Study Design: Prospective cohort study.
Methods: Surgical specimens collected from 20 cholesteatomas and nine COMs were treated to dissolve biofilms and subjected to 16S ribosomal RNA (rRNA) gene sequencing and amplicon sequence variant-level analysis for microbiota profiling and quantitative comparison. Correlations between the relative abundance of potential pathogens and the volume of the primary resected cholesteatomas were examined.
Results: Differences in bacterial composition (beta diversity) were observed between cholesteatomas and COM ( = .002), with a higher abundance of in cholesteatomas than in COM ( = .005). Common genera in the external auditory canal (EAC) flora, such as , , and , were predominant in both cholesteatoma and COM; and were increased in both diseases compared with the EAC flora. Furthermore, coagulase-negative staphylococci (CoNS) were more abundant in cholesteatomas than in COM ( = 0.002). Linear discriminant analysis coupled with effect size measurements (LEfSe) identified four CoNS as potential biomarkers for cholesteatoma. The relative abundance of , a potential pathogen, was positively correlated with cholesteatoma volume ( = .60, = .02).
Conclusion: The microbiota of cholesteatoma and COM originated from EAC flora, but the bacterial composition was largely altered. Our results suggested that infection is involved in cholesteatoma progression.
Level Of Evidence: 3b.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9764795 | PMC |
| http://dx.doi.org/10.1002/lio2.934 | DOI Listing |
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Article Synopsis
- - The study aimed to compare the microbiota of cholesteatomas and chronic suppurative otitis media (COM) to identify potential pathogens linked to cholesteatoma phenotypes.
- - Analysis of surgical specimens showed significant differences in bacterial composition between cholesteatomas and COM, with certain bacteria being more prevalent in cholesteatomas.
- - Findings indicate that coagulase-negative staphylococci (CoNS) could serve as biomarkers for cholesteatomas, and their abundance was positively correlated with the size of the cholesteatoma, suggesting their role in disease progression.
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