Objectives: This study aims to evaluate the anticancer activity of arginine-reduced graphene (Gr-Arg) and ginsenoside Rh2-containing arginine-reduced graphene (Gr-Arg-Rh2).
Materials And Methods: Thirty-two mice with breast cancer were divided into four groups and treated every three days for 32 days: Group 1, PBS, Group 2, Rh2, Group 3, Gr-Arg, and Group 4, Gr-Arg-Rh2. The tumor size and weight, gene expression (IL10, INF-γ, TGFβ, and FOXP3), and pathological properties of the tumor and normal tissues were assessed.
Results: Results showed a significant decrease in expression for all drug treatment groups compared with the controls (=0.04). There was no significant difference among the groups regarding and gene expression profiles (>0.05). Gr-Arg-Rh2 significantly inhibited tumor growth (size and weight) compared with Rh2 and control groups. The highest survival rate and the highest percentage of tumor necrosis (87.5%) belonged to the Gr-Arg-Rh2 group. Lungs showed metastasis in the control group. No metastasis was observed in the Gr-Arg-Rh2 group. Gr-Arg-Rh2 showed partial degeneration of hepatocytes and acute cell infiltration in the portal spaces and around the central vein. The Gr-Arg group experienced a moderate infiltration of acute cells into the port spaces and around the central vein. The Rh2 group also showed a mild infiltration of acute and chronic cells in portal spaces.
Conclusion: Based on the results, Gr-Arg-Rh2 can reduce tumor size, weight, and growth, TGF-β gene expression, and increase tumor necrosis and survival time in mice with cancer.
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http://dx.doi.org/10.22038/IJBMS.2022.66065.14524 | DOI Listing |
Iran J Basic Med Sci
December 2022
Faculty of Interdisciplinary Science and Technology, Tarbiat Modares University, Tehran, Iran.
Mikrochim Acta
August 2018
College of Chemistry and Chemical Engineering, Henan University, Kaifeng, 475004, China.
A glassy carbon electrode (GCE) was modified with poly(L-arginine) (P-Arg), reduced graphene oxide (rGO) and gold nanoparticle (AuNP) to obtain an electrode for simultaneous determination of dopamine (DA), serotonin (5-HT) and L-tryptophan (L-Trp) in the presence of ascorbic acid (AA). The modified GCE was prepared via subsequent 'layer-by-layer' deposition using an electrochemical technique. The surface morphology of the modified electrode was studied by scanning electron microscopy, and electrochemical characterizations were carried out via cyclic voltammetry and electrochemical impedance spectroscopy.
View Article and Find Full Text PDFBiosens Bioelectron
October 2018
College of Chemistry and Chemical Engineering, Henan University, China; Key Laboratory of Natural Medicine and Immune-Engineering of Henan Province, Kaifeng 475004, Henan, China. Electronic address:
An innovative electrochemical sensor assembly relying on a simple "green" electrochemical reduction route is presented for the sensitive detection of 8-hydroxy-2'-deoxyguanosine (8-OHdG), the most abundant oxidative product of DNA. The sensing film consisted of poly (L-arginine) and graphene wrapped Au nanoparticles was fabricated on glassy carbon electrode (GCE/P-Arg/ErGO-AuNPs) using subsequent 'layer-by-layer' regime through electrochemical technique. The proposed method was also successfully applied for the quantification of 8-OHdG in the presence of interfering biomolecules like ascorbic acid and uric acid.
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