AI Article Synopsis
- - The study investigated the presence and genetic traits of Cryptosporidium spp. in 100 patients with gastrointestinal (GI) cancer in Egypt, comparing them to 20 healthy controls.
- - Results showed that only 7% of cancer patients tested positive for Cryptosporidium using microscopy and a specific kit, but 40% were positive through a more sensitive genetic test, suggesting underreporting with traditional methods.
- - It was found that male sex and younger age were significant risk factors for infection, and researchers identified a new subtype of C. parvum, urging further studies to better understand its genetic characteristics.
Article Abstract
While the importance of cryptosporidiosis in immunocompromised persons is well known, the prevalence of Cryptosporidium spp. in cancer patients is not clear. The current study was designed to assess the occurrence and genetic characteristics of Cryptosporidium spp. in patients with gastrointestinal (GI) cancer in Egypt. Stool samples were collected from 100 patients with GI malignancies and 20 healthy individuals without any GI manifestations (control group). They were screened by microscopy and the immunochromatographic RIDA®QUICK Cryptosporidium kit. Subtyping of Cryptosporidium spp. was conducted by sequence analysis of the glycoprotein 60 (gp60) locus. Sociodemographic, environmental data and information on GI symptoms, cancer types, and clinical treatment were obtained via a questionnaire. By microscopy and RIDA®QUICK, only 7% (7/100) of GI cancer patients were positive for Cryptosporidium, compared with 40% (40/100) by gp60 nPCR. No positives were obtained from the control group. Male sex (P = 0.02) and younger age (P = 0.004) were major Cryptosporidium risk factors for infection. The occurrence of Cryptosporidium was also significantly more frequent (P = 0.003) in watery stool samples. Sequence analysis of the gp60 amplicons (~ 400 bp) identified a novel C. parvum subtype with nine TCA repeats and eleven ACATCA repeats. A formal subtype designation could not be made due to the short sequence length. More studies should be conducted to verify the common occurrence of this unusual C. parvum subtype and establish its genetic identity.
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| http://dx.doi.org/10.1007/s00436-022-07761-8 | DOI Listing |
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