AI Article Synopsis
- Researchers developed a method to effectively assemble complex DNA origami structures by using perfluorinated tags that help guide the pieces together through a process called fluorous-directed recognition.
- By combining this with traditional Watson-Crick base-pairing techniques, they achieved a significant increase (8 times higher yield) in the creation of specific DNA constructs compared to using each method separately.
- This new "catch-and-latch" strategy not only boosts assembly efficiency but also reduces the number of DNA sequences needed, making it a cost-effective and influential approach in the field of DNA nanotechnology.
Article Abstract
An orthogonal, noncovalent approach to direct the assembly of higher-order DNA origami nanostructures is described. By incorporating perfluorinated tags into the edges of DNA origami tiles we control their hierarchical assembly via fluorous-directed recognition. When we combine this approach with Watson-Crick base-pairing we form discrete dimeric constructs in significantly higher yield (8x) than when either molecular recognition method is used in isolation. This integrated "catch-and-latch" approach, which combines the strength and mobility of the fluorous effect with the specificity of base-pairing, provides an additional toolset for DNA nanotechnology, one that enables increased assembly efficiency while requiring significantly fewer DNA sequences. As a result, our integration of fluorous-directed assembly into origami systems represents a cheap, atom-efficient means to produce discrete superstructures.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9835977 | PMC |
| http://dx.doi.org/10.1021/acsnano.2c10727 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!