Novel pyrimido-pyridazine derivatives: design, synthesis, anticancer evaluation and studies.

A novel pyrimido-pyridazine derivative for developing anticancer agents was synthesized via Ullmann arylation using an efficient Cu(OAc) catalyst. Compounds were investigated for their anticancer potential, against human breast adenocarcinoma cells, viz. MCF-7, MDA-MB-231 and normal cell line HEK-293. Further, an study was conducted on lymphoma-bearing mice while analysis was carried out for molecular interactions. Compound displayed significant antitumor activity towards MDA-MB-231 cells through induction of apoptosis and arresting cells in S-phase , while it significantly increased the lifespan and reduced tumor growth . An  study revealed potent tyrosine-protein kinase inhibitors. Taken together the molecule has the potential to become an effective therapeutic treatment for breast cancer.