AI Article Synopsis
- Small RNAs like miRNAs and hc-siRNAs play a crucial role in gene regulation and were explored in the context of two potato cultivars, Desiree and Eva.
- The study established a small RNA expression atlas across various potato tissues (leaves, flowers, roots, and tubers) and found that most small RNAs were conserved between the cultivars, suggesting a low evolutionary rate.
- Some miRNAs displayed differences in accumulation between cultivars, and certain hc-siRNAs showed tissue-specific expression, highlighting the complexity of small RNA regulation in potatoes.
Article Abstract
Small RNAs, including microRNAs (miRNAs), phased secondary small interfering RNAs (phasiRNA), and heterochromatic small interfering RNAs (hc-siRNA) are an essential component of gene regulation. To establish a broad potato small RNA atlas, we constructed an expression atlas of leaves, flowers, roots, and tubers of Desiree and Eva, which are commercially important potato () cultivars. All small RNAs identified were observed to be conserved between both cultivars, supporting the hypothesis that small RNAs have a low evolutionary rate and are mostly conserved between lineages. However, we also found that a few miRNAs showed differential accumulation between the two potato cultivars, and that hc-siRNAs have a tissue specific expression. We further identified dozens of reproductive and non-reproductive phasiRNAs originating from coding and noncoding regions that appeared to exhibit tissue-specific expression. Together, this study provides an extensive small RNA profiling of different potato tissues that might be used as a resource for future investigations.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9751654 | PMC |
| http://dx.doi.org/10.1002/pld3.466 | DOI Listing |
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