AI Article Synopsis
- The study explored ways to make intracytoplasmic sperm injection (ICSI) more accessible by optimizing the technique for efficiency and practicality.
- Trypsin treatment was effective in removing mouse sperm tails, leading to a successful use of pre-activated oocytes that matched the fecundity of untreated sperm while improving durability and injection time.
- Similar success was observed in rats, where the new method not only increased birth rates but also streamlined the ICSI process for broader applicability in various research labs.
Article Abstract
We examined various methods to enhance the accessibility of intracytoplasmic sperm injection (ICSI) technology to more users by making the technique easier, more efficient, and practical. First, the methods for artificially removing the mouse sperm tail were evaluated. Trypsin treatment was found to efficiently remove the sperm tails. The resultant sperm cells had a lower oocyte activation capacity; however, the use of activated oocytes resulted in the same fecundity as that of fresh, untreated sperm. Pre-activated oocytes were more resistant to physical damage, showed higher survival rates, and required less time per injection. Testing this method in rats yielded similar results, although the oocyte activation method was different. Remarkably, this method resulted in higher birth rates of rat progeny than with conventional methods of rat ICSI. Our method thereby streamlines mouse and rat ICSI, making it more accessible to laboratories across many disciplines.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9939287 | PMC |
| http://dx.doi.org/10.1262/jrd.2022-065 | DOI Listing |
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