AI Article Synopsis
- The study examines how different carbon sources (glucose, olive oil, and a mix of both) affect the growth and enzyme production of the fungus Penicillium simplicissimum using two fermentation methods: submerged and solid-state.
- In solid-state fermentation, the fungus grows both on and inside an inert support, showing greater biomass and enzyme production under stressful conditions, while submerged fermentation leads to spherical pellets with optimal yields observed with the combined glucose and olive oil medium.
- The results suggest that the production process not only enhances lipase output but also allows for the potential direct application of the enzyme without additional processing steps.
Article Abstract
The influence of different carbon sources (glucose (G), olive oil (O), and a combination of both (GO)) in the physiology (biomass and lipase production) and morphology (light and environmental and scanning electron microscopy) of the fungus Penicillium simplicissimum by applying submerged (SmF) and solid-state (SSF) fermentations was investigated. The cultivation was carried out using polypropylene as hydrophobic inert support in SmF and SSF to understand better the influence of a support for the fungus growth and also provides the immobilization of lipases during its production. Micrographs show different morphologies: in SSF, the fungus grows on and inside the inert support independent of the media; in SmF, the formation of high-density spherical pellets obtained in medium GO leads to the best productivity and specific product yield Y.Conidiation is observed mainly in SSF, a few in SmF with polypropylene as inert support and not in SmF, which may indicate a stress condition in SSF. Possibly, the morphology acquired by the fungus under stressful conditions may be the key to the higher biomass and lipase productivity at SSF. The developed process with simultaneous production and immobilization of lipase leads to a new promissory biocatalyst once it can be directly applied with no need for downstream processes.
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| http://dx.doi.org/10.1016/j.enzmictec.2022.110173 | DOI Listing |
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