AI Article Synopsis
- A protocol is presented to identify immunogenic self-peptide/allogeneic MHC epitopes by utilizing alloreactive CD8+ T cells.
- Mice are primed with a skin graft containing an allogeneic MHC class I molecule, then boosted with a liver-specific AAV vector carrying that MHC's heavy chain.
- A peptide-exchange method is employed to create peptide-MHC multimers for evaluating T cell recognition of specific epitopes.
Article Abstract
Here, we present a protocol to identify immunogenic self-peptide/allogeneic major histocompatibility complex (MHC) epitopes. We describe the generation of enriched alloreactive CD8+ T cells by priming mice with a skin graft expressing the allogeneic MHC class I molecule of interest, followed by boosting with a liver-specific AAV vector encoding the heavy chain of that donor MHC allomorph. We then use a peptide-exchange approach to assemble a range of peptide-MHC (pMHC) multimers for measuring recognition of the various epitopes by these alloreactive T cells. For complete details on the use and execution of this protocol, please refer to Son et al. (2021)..
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9792548 | PMC |
| http://dx.doi.org/10.1016/j.xpro.2022.101943 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Immunopeptidomics Mapping of Listeria monocytogenes T Cell Epitopes in Mice.
Mol Cell Proteomics
September 2024
VIB-UGent Center for Medical Biotechnology, VIB, Ghent, Belgium; Department of Biomolecular Medicine, Ghent University, Ghent, Belgium; VIB Proteomics Core, VIB, Ghent, Belgium. Electronic address:
Listeria monocytogenes is a foodborne intracellular bacterial model pathogen. Protective immunity against Listeria depends on an effective CD8 T cell response, but very few T cell epitopes are known in mice as a common animal infection model for listeriosis. To identify epitopes, we screened for Listeria immunopeptides presented in the spleen of infected mice by mass spectrometry-based immunopeptidomics.
View Article and Find Full Text PDFFifty years after the discovery of the association of HLA B27 with ankylosing spondylitis.
RMD Open
August 2023
Department of Rheumatology, Charite Universitatsmedizin Berlin, Berlin, Germany.
The human lymphocyte antigen B27 (HLA B27) is a member of the HLA class I family of genes in the major histocompatibility complex whose name goes back to its discovery in studies of transplanted tissue compatibility. Its prevalence in the mid-European population is about 8%. The association of HLA B27 alleles with ankylosing spondylitis (AS), a highly heritable disease, which is part of the spectrum of axial spondyloarthritis (axSpA), was discovered 50 years ago.
View Article and Find Full Text PDFImmunoglobulin E autoantibodies in atopic dermatitis associate with Type-2 comorbidities and the atopic march.
Allergy
December 2023
Skin Immunology & Immune Tolerance (SKIN) Research Group, Vrije Universiteit Brussel (VUB), Brussels, Belgium.
Background: Autoreactive immunoglobulin E (IgE) antibodies to self-peptides within the epidermis have been identified in patients with atopic dermatitis (AD). Prevalence, concomitant diseases, patient characteristics, and risk factors of IgE autoantibody development remain elusive. We aimed to determine IgE autoantibodies in serum samples (n = 672) from well-characterized patients with AD and controls (1.
View Article and Find Full Text PDFA Challenging Diagnosis of Febrile Pancytopenia in a Patient With a History of Autoimmune Disease.
Cureus
March 2023
Medicine/Infectious Diseases, St. Barnabas Hospital Health System, Bronx, USA.
Pancytopenia is a hematologic condition characterized by a decrease in all three peripheral blood cell lines. There are many causes of pancytopenia, and the proper approach is required for accurate diagnosis. Brucellosis and systemic lupus erythematosus (SLE) are both diseases that can initially present as pancytopenia, both of which require a targeted workup to diagnose.
View Article and Find Full Text PDFImmunogenic self-peptides - the great unknowns in autoimmunity: Identifying T-cell epitopes driving the autoimmune response in autoimmune diseases.
Front Immunol
January 2023
Department of Dermatology, University Clinics, Ludwig-Maximilian-University of Munich, Munich, Germany.
HLA-associated autoimmune diseases likely arise from T-cell-mediated autoimmune responses against certain self-peptides from the broad HLA-presented immunopeptidomes. The limited knowledge of the autoimmune target peptides has so far compromised the basic understanding of autoimmune pathogenesis. This is due to the complexity of antigen processing and presentation as well as the polyspecificity of T-cell receptors (TCRs), which pose high methodological challenges on the discovery of immunogenic self-peptides.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!