AI Article Synopsis

  • SIP helps track labeled substrates in fungi, enhancing our understanding of their ecological role and environmental relationships.
  • Combining stable isotope probing with rRNA and mRNA analysis reveals connections between microbial diversity and function.
  • The described methods aim to study carbon transfer between plants and their mycorrhizal partners, shedding light on their interactions.

Article Abstract

The use of stable-isotope probing (SIP) allows tracing specific labeled substrates into fungi leading to a better understanding of their role in biogeochemical cycles and their relationship with their environment. Stable isotope probing combined with ribosomal RNA molecule, conserved in the three kingdoms of life, and messenger RNA analysis permits the linkage of diversity and function. Here, we describe two methods designed to investigate the interactions between plants and their associated mycorrhizal compartment by tracing carbon flux from the host plant to its symbionts.

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Source
http://dx.doi.org/10.1007/978-1-0716-2871-3_9DOI Listing

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