Background: () family genes are known to be involved in pathogen induced plant responses to biotic stress. Even though the family genes have been identified and characterized in plant defense responses in some plants, the roles of these genes associated with the plant abiotic stress tolerance in wild soybean is not fully established yet, especially in response to alkaline stress.
Methods: We identified the potential family genes by using the Hidden Markov model and wild soybean genome. The maximum-likelihood phylogenetic tree and conserved motifs were generated by using the MEME online server and MEGA 7.0 software, respectively. Furthermore, the syntenic analysis was generated with Circos-0.69. Then we used the PlantCARE online software to predict and analyze the regulatory -acting elements in promoter regions. Hierarchical clustering trees was generated using TM4: MeV4.9 software. Additionally, the expression levels of family genes under alkaline stress, ABA and MEJA treatment were identified by qRT-PCR.
Results: In this study, we identified 59 potential family genes in wild soybean. We identified that wild soybean family genes could be mainly classified into five groups as well as exist with conserved motifs. Syntenic analysis of family genes revealed genes location on 18 chromosomes and presence of 65 pairs of duplication genes. Moreover, family genes consisted of a variety of putative hormone-related and abiotic stress responsive elements, where numbers of methyl jasmonate (MeJA) and abscisic acid (ABA) responsive elements were significantly larger than other elements. We confirmed the regulatory roles of family genes in response to alkaline stress, ABA and MEJA treatment. In conclusion, we identified and provided valuable information on the wild soybean family genes, and established a foundation to further explore the potential roles of family genes in crosstalk with MeJA or ABA signal transduction mechanisms under alkaline stress.
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| http://dx.doi.org/10.7717/peerj.14451 | DOI Listing |
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