AI Article Synopsis

  • Diabetic kidney disease (DKD) is a major global health issue, and there's a need for better noninvasive methods to predict its progression in diabetic patients.
  • The study measured SHP-1 levels in kidney tissues, urinary podocytes, and blood from 66 patients (48 diabetic and 18 nondiabetic), finding that diabetes increased SHP-1 expression in the kidneys.
  • The results suggest that urinary podocyte SHP-1 levels could serve as a useful marker for monitoring kidney disease progression in diabetic individuals.

Article Abstract

Background: Diabetic kidney disease (DKD) remains the leading cause of end stage kidney disease worldwide. Despite significant advances in kidney care, there is a need to improve noninvasive techniques to predict the progression of kidney disease better for patients with diabetes. After injury, podocytes are shed in urine and may be used as a biologic tool. We previously reported that SHP-1 is upregulated in the kidney of diabetic mice, leading to podocyte dysfunction and loss. Our objective was to evaluate the expression levels of SHP-1 in urinary podocytes and kidney tissues of patients with diabetes.

Methods: In this prospective study, patients with and without diabetes were recruited for the quantification of SHP-1 in kidney tissues, urinary podocytes, and peripheral blood monocytes. Immunochemistry and mass spectrometry techniques were applied for kidney tissues. Urinary podocytes were counted, and expression of SHP-1 and podocyte markers were measured by quantitative PCR.

Results: A total of 66 participants (diabetic =48, nondiabetic =18) were included in the analyses. Diabetes was associated with increased SHP-1 expression in kidney tissues (=0.03). Nephrin and podocin mRNA was not significantly increased in urinary podocytes from patients with diabetes compared with those without diabetes, whereas levels of SHP-1 mRNA expression significantly correlated with HbA1c and estimated glomerular filtration rate (eGFR). Additionally, follow-up (up to 2 years post recruitment) evaluation indicated that SHP-1 mRNA expression continued to increase with eGFR decline.

Conclusions: Levels of SHP-1 in urinary podocytes may serve as an additional marker of glomerular disease progression in this population.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9717659PMC
http://dx.doi.org/10.34067/KID.0002152022DOI Listing

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